Whole genome DNA sequencing kits
A wide range of library preparation kits are available to suit all whole genome sequencing requirements. Amplification-free kits allow direct, long-read sequencing of native DNA, eliminating the potential for PCR bias and allowing the detection of base modifications alongside nucleotide sequence. Amplification-based kits are also available, enabling whole genome sequencing from low input amounts or poor quality DNA (e.g. FFPE).
| Ligation Sequencing Kit | Ligation Sequencing Kit | Ultra-Long DNA Sequencing Kit | Rapid PCR Barcoding Kit | |
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| Preparation time | 60 min | 10 min | 60 min + ON elution | 15 min + PCR |
| Input requirement | 1000 ng of gDNA or 100-200 fmol of amplicons or cDNA | 100-400 ng HMW gDNA |
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1-5 ng gDNA |
| Fragmentation | Optional | Transposase based | Transposase based | Transposase based |
| Read length | Equal to fragment length | Random distribution, dependent on input fragment length | 50-100+ kb N50 (R9.4.1 flow cells) | ~2-5 kb |
| Typical throughput |
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| Multiplexing options | Yes | Yes | In development | This kit offers barcoding for up to twelve samples |
| Overview | Optimised for throughput; retained base modifications; control over read length; compatible with whole genome amplification | Simple and rapid; retained base modifications; cold chain-free (Field kit) | Optimised for production of ultra-long reads (N50 ≥50 kb); retained base modifications | Simple and rapid |