Targeted DNA sequencing kits
Long-read nanopore sequencing expands the application of targeted sequencing to include cost-effective, assembly-free analysis of repetitive regions, structural variants, and variant phasing across entire genes or large genomic regions of interest. Through analysis of the entire 16S rRNA gene, the long sequencing reads provided by the 16S Barcoding Kit also provide more accurate taxonomic classification of bacteria and archaea.
| Ligation Sequencing Kit | Cas9 Sequencing Kit | 16S Barcoding Kit | ||
|---|---|---|---|---|
| Approach | Amplicon | Sequence capture | CRISPR/Cas9 | Amplicon |
| Preparation time | 60 min | >24 hours | 110 min | 10 min + PCR |
| Input requirement | 100-200 fmol amplicon | 3.5 µg HMW gDNA | 1-10 µg HMW gDNA (5 µg recommended) | < 10 ng gDNA |
| Fragmentation | N/A | Optional | Cas9-dependent cleavage | N/A |
| Read length | Equal to fragment length | Equal to fragment length post-PCR | Equal to fragment length | Full-length 16S gene (~1.5kb) |
| Multiplexing options | Yes | Yes | In development | This kit offers barcoding for up to twelve samples |
| Typical throughput |
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| Multiplexing options | Yes | No | No | Yes |
| Overview | Simple, high-throughput workflow for existing amplicons | Compatible with sequence capture approaches | Streamlined workflow; preservation of base modifications | Precise, real-time identification of bacteria and archaea |