RNA sequencing kits
Confidently characterise and quantify full-length RNA transcripts, splice variants, and fusions using long-read nanopore sequencing. Accurately analyse differential gene expression and transcript usage. Sequence native RNA directly, without amplification or reverse transcription, and identify base modifications.
| Direct RNA Sequencing Kit | cDNA-PCR Sequencing Kit | |||
|---|---|---|---|---|
| Preparation time | 105 min | 165 min | ||
| Input requirement | 500 ng total RNA or 50ng poly-A+ RNA | 4 ng poly(A)+ RNA or 200 ng total RNA | ||
| Reverse transcription required | Optional | Yes | ||
| PCR required | No | Yes | ||
| Read length | Equal to RNA length | Enriched for full-length cDNA | ||
| Typical throughput |
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| Multiplexing options | In development | Yes | ||
| Overview | Sequencing RNA molecules directly; identify base modifications and poly-A tail length | Optimised for throughput | ||