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AriaMx Real-Time PCR (qPCR) Instrument

AriaMx Real-time PCR System

The AriaMx Real-Time PCR system is a fully integrated qPCR solution for amplification, detection, and data analysis, combining a novel thermal cycler, an advanced optical system with LED excitation source, and comprehensive data analysis software.

AriaMx has a closed-tube PCR detection format and can be used with many fluorescence detection chemistries, including SYBR Green and EvaGreen dyes, and fluorogenic probe systems such as TaqMan. Functional reporting, more than 120 attributes, and built-in diagnostics assist in troubleshooting. A modular and flexible design with intuitive touch-screen interface improves productivity.

Features

  • Usable with many fluorescence detection chemistries, including SYBR Green and EvaGreen dyes, and fluorogenic probe systems such as TaqMan
  • Combines a novel thermal cycler, an advanced optical system with LED excitation source, and comprehensive data analysis software
  • Ultrafast chemistry helps you to complete your study in a timely fashion
  • Proprietary rapid hot-start enzymes and fastest industry high-resolution melt reagents
  • Intuitive operation with a touch screen interface
  • Modular optics and open reagent platform for increased agility
  • Multiplex capability with six filters for five plexing and two to five channels
  • Validated for most applications and chemistries
  • Intuitive open-source software and robust data analysis with proprietary algorithms
  • Instrument validated on 100,000+ samples across 14 different assays

Specifications

ARIA Software - Standard with Instrument Yes
Accuracy ±0.2 °C
Controller Touchscreen Yes
Data Acquisition Time < 3 sec
Detection Sources 8 filtered photodiodes
Dimensions (WxDxH) 50.0 x 46.0 x 42.0 cm (19.7 x 18.1 x 16.5 inches)
Dynamic Range 9
Excitation Source 8 dye specific LEDs per optical module
Max Optical Modules 6
Maximum Block Ramp Rate 6 °C/Sec
Number of Peltiers 6
Number of Wells 96
Number of wells for multiplexing Up to 5 targets per well
Optical Cartridge Types
SYBR/FAM ROX HEX
CY5 CY3 ATTO425
PCR Uniformity ±0.4 °C
Protocol Storage Count 5GB
Reaction Volume 10-30 µL
Remote Monitoring Yes
Self-Installable Optical Cartridges Yes
Software Included Free software including LIMS connectivity feature 21 CFR part 11 enabled software
Software Upgrade Statements Available online, no charge
Standard Warranty Length 1 yr
Thermal System Temperature Range 25.0-99.9 °C

Featured References

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  2. Kim, K., Kwon, O., Ryu, T. Y., Jung, C., Kim, J., Min, J., Kim, D., Son, M., Cho, H. Propionate of a microbiota metabolite induces cell apoptosis and cell cycle arrest in lung cancer. Molecular Medicine Reports, 2019. 20, 1569-1574
  3. Lass, A., Ma, L., Kontogeorgos, I. et al. First molecular detection of Toxoplasma gondii in vegetable samples in China using qualitative, quantitative real-time PCR and multilocus genotyping. Sci Rep 9, 17581 (2019).
  4. Beims H, Janke M, von der Ohe W, Steinert M. Rapid identification and genotyping of the honeybee pathogen Paenibacillus larvae by combining culturing and multiplex quantitative PCR. Open Vet J. 2020;10(1):53-58. doi:10.4314/ovj.v10i1.9
  5. Valledor, S., Valledor, I., Gil-Rodríguez, M.C. et al. Comparison of several Real-Time PCR Kits versus a Culture-dependent Algorithm to Identify Enteropathogens in Stool Samples. Sci Rep 10, 4301 (2020).
  6. Page-Karjian A, Chabot R, Stacy NI, Morgan AS, et al. Comprehensive health assessment of green turtles Chelonia mydas nesting in southeastern Florida, USA. Endang Species Res 42:21-35 (2020)
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  9. Yohko Yamaguchi, Kohei Kaida, Yusuke Suenaga, Akihito Ishigami, Yoshiro Kobayashi, Kisaburo Nagata. Age-related dysfunction of p53-regulated phagocytic activity in macrophages, Biochemical and Biophysical Research Communications, Volume 529, Issue 2, 2020, Pages 462-466, ISSN 0006-291X,
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  11. Marnis, H; Kania, P W; Syahputra, K; Zuo, S; Buchmann, K. Local immune depression in Baltic cod (Gadus morhua) liver infected with Contracaecum osculatum. Journal of Helminthology; Cambridge Vol. 94, (2020). DOI:10.1017/S0022149X19001111
  12. Egorova M.S., Kurashova S.S., Ishmukhametov A.A., Balovneva M.V., Deviatkin A.A., Safonova M.V., Ozherelkov S.V., Khapchaev Yu.K., Balkina A.S., Belyakova A.V., Dzagurova T.K., Tkachenko E.A. Real-time PCR assay development for the control of vaccine against hemorrhagic fever with renal syndrome. Problems of Virology. 2021;66(1):65-73
  13. Link, E.K., Eddicks, M., Nan, L. et al. Discriminating the eight genotypes of the porcine circovirus type 2 with TaqMan-based real-time PCR. Virol J 18, 70 (2021).
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